Saghi Hakimi Naeini
Shahid Beheshti University, Iran
Title: The protective effect of glycitin on pentylenetetrazole-induced seizures in male wistar rats
Biography
Biography: Saghi Hakimi Naeini
Abstract
Introduction:
Epilepsy is a neurological disorder affecting millions of people and causes recurring seizures. Glycitin is a flavonoid found in soybeans and have various pharmacological properties, including anti-inflammatory, antioxidant, and neuroprotective effects. However, the effects of glycitin on pentylenetetrazole (PTZ)-induced seizures in Wistar rats have not been extensively investigated. In this study, we investigated the potential anticonvulsant effect of glycitin in a rat model of PTZ-induced seizures.
Materials and methods :
Adult Wistar rats were obtained from Shahid Beheshti Medical University. Rats were kept under constant conditions. PTZ and glycitin were purchased from Sigma, USA. A total of 36 male Wistar rats were divided into 6 groups with 6 rats in each group. The first group received saline only, while the second group received saline followed by PTZ (35 mg. kg−1) after 30 minutes. The third to six received phenobarbital (30 mg. kg−1) and glycitin (5, 10, and 20 mg. kg−1) respectively, followed by PTZ after 30 minutes. All injections were administered once every 48 hours for a duration of 21 days via intraperitoneal route. The severity of seizures was assessed using a score adapted from the Racine score, which ranges from 0 to 5. Seizures were recorded for a period of 30 minutes after PTZ injection.
Results:
The analysis of seizure scores showed that the groups receiving glycitin had delayed and less severe seizures compared to the group receiving PTZ after saline. Specifically, the group receiving 5 mg. kg−1 of glycitin showed the most significant reduction in seizure score compared to the PTZ group.
Conclusion:
This study demonstrates that glycitin has anticonvulsant effects in a PTZ-induced seizure model in male Wistar rats. The delayed and less severe seizures observed in the groups receiving glycitin. Further studies are needed to determine the mechanisms underlying its anticonvulsant effects.